qPCR kits

What is different in Biotical qPCR kits?

  • All our qPCR kits are produced in the UE (SPAIN), 
  • CE marked and validated under the new EU regulatory mark of sanitary products.
  • From 1 to 96 samples per assay.
  • Ready-to-use and easy-to-use kits
  • High sensitivity, specificity and reproducibility
  • All our products are LYOPHILIZATED:
  • NO COLD CHAIN REQUIRED. The product is stable at 25ºC.
  • 24 Months of expiry date.

We offer a flexible service, with several options for customization:

  • 96 Strips or 48 Strips option in all references
  • Low Strip, high Strip or Tube
  • 3 types of controls available:

INTERNAL CONTROL

It is a parallel reaction ran in the same reaction well with the same PCR reagents except primer and probe, the reaction is followed in different fluorescence channel (HEX or Cy5).

IC serves to verify the PCR assay:

  • The reaction well has properly rehydrated
  • The reagents are in proper condition
  • The PCR system has executed the thermal cycle and the optical system is working

EXTRACTION CONTROL

EC (green vial) is a non-infectious nucleic acid lyophilized with the sequence. EC HEX or Cy5 channel. Extraction Control can be used to monitor nucleic acid isolation and/or as PCR inhibition control.

ENDOGENOUS CONTROL

It is a parallel reaction ran in the same reaction well with the same PCR reagents except primer and probe. Housekeeping gene (human RNase P gene). Human housekeeping genes are involved in basic cell maintenance and, therefore, are expected to be present in all nucleated human cells and maintain relatively constant expression levels. 

Thermal Cycling Protocol for DNA:

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