What is different in Biotical qPCR kits?
We offer a flexible service, with several options for customization:
It is a parallel reaction ran in the same reaction well with the same PCR reagents except primer and probe, the reaction is followed in different fluorescence channel (HEX or Cy5).
IC serves to verify the PCR assay:
EC (green vial) is a non-infectious nucleic acid lyophilized with the sequence. EC HEX or Cy5 channel. Extraction Control can be used to monitor nucleic acid isolation and/or as PCR inhibition control.
It is a parallel reaction ran in the same reaction well with the same PCR reagents except primer and probe. Housekeeping gene (human RNase P gene). Human housekeeping genes are involved in basic cell maintenance and, therefore, are expected to be present in all nucleated human cells and maintain relatively constant expression levels.